Mr Geoff Osborne

Contact Information

g.osborne@uq.edu.au
Building: QBI Building #79
Room: 532
Tel: +61 7 334 66397

Mailing Address

Queensland Brain Institute
The University of Queensland
Brisbane, 4072
Queensland
Australia

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Lab Members

Lab Home Page

Short biography

Research directions

Current collaborations

Selected publications

 

Short biography

Mr Osborne obtained an Associate Diploma in Applied Biology in 1983, before a Bachelor of Science from the Australian National University (ANU). He began specialising in flow cytometry in 1989 at the John Curtin School of Medical Research, ANU. From 1989 to 1999 his interest lay in developing novel flow cytometry technologies in software and hardware with a particular emphasis cell sorting and high content screening applications. In 2000 he moved to the University of British Columbia, Vancouver, Canada to establish a core facility before return to ANU in 2001.

He was recruited in 2004 to a joint position at The Queensland Brain Institute and the Australian Institute for Bioengineering and Nanotechnology.

Research directions

Flow cytometry is a cornerstone technology for the study of haematopoietic stem cell function and cellular immunology in general. This is due not only to its ability to analyse and sort specific populations of stem, progenitor and more differentiated blood cell types, but also because it can provide quantitative information pertaining to viability, DNA content, mitotic activity and protein expression patterns of specific cell types. Given these strengths it is surprising that flow cytometry has previously had little traction in neuroscience. This laboratory seeks to combine these strengths with recent advances that allow the purification of essentially pure populations of viable neural stem cells, neural crest cells and post-mitotic neurons using flow cytometry, to explore new avenues for research.

Currently Mr Osborne’s research interests involve novel implementations of flow technology in neuroscience, in particular the role of flow cytometry can play in elucidating the biology of brain tumours and stem cells.

Current collaborations

Selected publications

Recent advances in flow cytometric cell sorting. GW Osborne. Methods Cell Biol, Jan 2011; 102: 533-56.

Purification of immature neuronal cells from neural stem cell progeny. H Azari, GW Osborne, T Yasuda, MG Golmohammadi, M Rahman, LP Deleyrolle, E Esfandiari, DJ Adams, B Scheffler, DA Steindler, and BA Reynolds PLoS One, January 1, 2011; 6(6): e20941.

Evidence for label-retaining tumour-initiating cells in human glioblastoma. Deleyrolle LP, Harding A, Cato K, Siebzehnrubl FA, Rahman M, Azari H, Olson S, Gabrielli B, Osborne G, Vescovi A, Reynolds BA (2011 Brain May 2011; 134: 1331 - 1343.

A method of quantifying cell sorting yield in "real time". Osborne G (2010) Cytometry A 77:983-989.

Cellular plasticity of inflammatory myeloid cells in the peritoneal foreign body response. Mooney J, Rolfe B, Osborne G, Sester D, van Rooijen N, Campbell G, Hume D, Campbell J (2010) Am J Pathol 176:369-380.